August 2025 DOT Compliance Updates: What Employers Must Know

As we move further into 2025, the Department of Transportation (DOT) continues to refine and implement regulations that impact employers across trucking, aviation, rail, transit, and other DOT-regulated industries. These updates aren’t just bureaucratic changes; they shape how companies manage drivers, maintain safety records, handle testing, and stay eligible to operate. Staying current is essential not only for compliance but for protecting business continuity and avoiding costly penalties.

Whether it’s understanding the evolving physical standards for driver medical certification, navigating the unique Alaska transportation laws that complement federal mandates, or simply seeking comprehensive support for your occupational health needs, MedPhysicals Plus is dedicated to keeping you informed and compliant.

DOT Drug and Alcohol Testing Requirements for 2025

Drug and alcohol testing remains central to DOT compliance. For 2025, random testing rates remain unchanged for most agencies, including FMCSA (50% drug, 10% alcohol), FAA, FRA, and FTA. However, PHMSA’s rate increased to 50% for drug testing in 2025, up from 25% in 2024. Employers must ensure their random testing programs stay aligned with these federal minimums year-round.

Is Oral Fluid Drug Testing Allowed for DOT?

Oral fluid testing was approved by the DOT in 2023 as an alternative to urine-based drug testing. However, it remains unavailable until two HHS-certified labs are approved, one for the primary test and one for the split specimen. As of August 2025, no oral fluid labs are certified, per SAMHSA’s July lab list (PDF) and the Federal Register’s certified lab listing. Until then, all DOT drug testing must still use urine samples.

FMCSA Compliance Changes: What Carriers Need to Know

The FMCSA has introduced several regulatory changes in 2025. Among the most impactful is the elimination of Motor Carrier (MC) numbers, effective October 1, 2025. Moving forward, USDOT numbers will be the sole identifier for all motor carriers. This shift is intended to reduce fraud and simplify compliance, so carriers should ensure they are set up to manage accounts via Login.gov.

FMCSA Medical Certification and Reporting Requirements

Starting June 23, 2025, certified medical examiners must transmit DOT physical exam results electronically to FMCSA’s National Registry within 24 hours. This update eliminates the need for drivers to carry paper medical cards. Employers and medical providers must ensure they’re using the current forms: the Medical Examination Report (MCSA-5875) and Medical Examiner’s Certificate (MCSA-5876), both updated in April 2025 (PDF). Current clinical guidance comes from the Medical Examiner’s Handbook (2024 Edition).

New Deregulatory FMCSA Rules in 2025

FMCSA has also rolled out several deregulatory changes aimed at reducing unnecessary administrative burden:

  • Accident reporting criteria have been revised to exclude diagnostic imaging from “medical treatment.”

  • CDL holders no longer need to self-report moving violations.

  • Electronic Driver Vehicle Inspection Reports (DVIRs) are now fully accepted.

  • Drivers no longer need to carry a paper ELD manual in the cab, as long as digital access is available.

DOT Compliance Support in Alaska with MedPhysicals Plus

For Alaska-based carriers and DOT-regulated employers, MedPhysicals Plus provides services that help keep operations in line with current federal requirements. From DOT physicals to drug and alcohol testing and occupational health services, the team supports compliance with streamlined scheduling and documentation. With convenient locations in Anchorage, Fairbanks, Wasilla, Soldotna, and Juneau, staying compliant is easier than ever.

Don’t let DOT regulations become a burden. Partner with MedPhysicals Plus to ensure your business remains compliant, efficient, and safe. We’re here to provide the expert support you need, precisely when you need it.